Hi Sonja, According to the Clontech website (http://www.clontech.com/archive/OCT99UPD/RFP.html), the emission of RedFP is 583nm so it should be possible. If it is possible it may be an idea to change the GFP collection filter to something a little lower as the emission is 508nm (Should be possible if you are using a sorter or a FACSCalibur). I have no direct experience of this, but did do some work with the YFP which again was supposedly possible to use in combination with GFP but found it almost impossible due to the amount of spectral overlap; however, RFP may be a better bet. The quantum yield allegedly isn't as good as EGFP so I (and I am sure others) would be interested to hear how you get on. Good luck, Derek On Fri, 7 Jan 2000, Sonja Rotzoll wrote: > I want to detect by flow cytometry cells transfected with 2 vectors the > one containing the GFP protein and the other the new Red fluorescence > protein from Clontech (humanized Red1). Do you know whether it is possible > to measure them with this combination of filters: > PI 585/42 for reFP and > FITC 530/30 for GFP ************************************************************************ Derek Davies Voice: (44) 0171 269 3394 FACS Laboratory, FAX: (44) 0171 269 3100 Imperial Cancer Research Fund, e_mail: derek.davies@icrf.icnet.uk London, UK Web Page: http://www.icnet.uk/axp/facs/davies/index.html In tenebris lux *************************************************************************
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