I got several good replies to my question re. the cell counts in invidual channels so have compiled them below. I have downloaded Ray Hicks software, FCSAssistant, and it does just what my PI wanted. Thanks to all who responded. Candace Enockson Medical University of South Carolina **** you can use my program, FCSPress, to do this. Moving the cursor along a histogram gives you a live display of channel number and cell count in the bottom left of the window. Alternatively you can export histogram data to a file for viewing in excel etc. using FCSPress, FCS Assistant, FlowJo and other programs. You can download a copy of FCSPress and FCS Assistant from http://www.FCSPress.com . If you've already tried a copy and your demonstration period has expired you can apply for an extension using the form at that site. Ray Hicks Cambridge. CB2 5QD UK **** I would assume if you placed both left and right marker in the same channel it would tell you the number of events in that channel. The other way would be to export the file in Binary format and then look at it in a wordpad or word program. I believe it will tell you the channel number and number of events. It is like the scroll on the 440 facs systems. Good luck, Mike Evans XY inc. **** (I think this is for Coulter Software?) You might try using the Copy Plot Data command under the Edit menu, and pasting the data into a Microsoft Excel spreadsheet. This will give you the y values for all 1024 channels (x values) of a one parameter histogram. Laura J. Jenski, Ph.D. Professor of Biology Department of Biology Indiana University-Purdue University at Indianapolis **** You can export CellQuest data into tab-delimited histogram format using Ray Hicks' FCS Assistant (available from http://facsmac.med.cam.ac.uk/). The resulting file has a .HD suffix and can be opened in either ClarisWorks (AppleWorks), Excel or CricketGraph (I think this may not be Y2K compliant though as it is a tad old now!) and presumably many other graphing programs. Once there you can easily get at the cell number per channel for each parameter. Derek Davies FACS Laboratory, Imperial Cancer Research Fund, London, UK **** Create and highlight a histogram of the data (gated or ungated) of interest and select the command save as FCS in the File menu (I think, as right now I am working on a PC, sigh!). This will create a file with two parameters: channel numbers and numbers of events. To read it, you can use Ray Hicks' software FCS Assistant. With FCS assistant you can also create a histogram file directly (use the save as Histogram command). Hope this helps. Regards Ulrik Sprogøe-Jakobsen Dept. Clinical Immunology Odense University Hospital
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:02 EST