Hi Maryalice- We process CSF specimens on a routine basis. We have published a study ( American Journal of Clinical Pathology. Sept. 1998 ) of 31 cases, one of which was an Anaplastic Large Cell Lymphoma. The total white cell count was 440,000 cells. We ran five 3-color tubes with the following markers: 45/5/7,45/19/30,45/5/cytoplasmic 3,3/4/8 ,45/5/30. The immunophenotypic results: An aberrant T cell population (surface CD3-,cytoplasmic CD3, CD7-, CD5+ and CD30+) consistent with T cell lymphoma. It would be difficult to make the diagnosis of ALCL in one tube. If we have greater than 20,000 cells we would run additional tubes/markers. Hope this helps. Clinical Flow Lab Northwestern Memorial Hospital Chicago,IL > -----Original Message----- > From: Maryalice Stetler-Stevenson [SMTP:stetler@box-s.nih.gov] > Sent: Tuesday, January 04, 2000 2:56 PM > To: Cytometry Mailing List > Subject: Anaplastic large cell > > Imagine you just receive a CSF and have enough cells to do one tube > (no I don't mean a million cells, I mean thousands). The request indicates > the patient has anaplastic large cell lymphoma and they want you to rule > out CNS involvement. What would you run? What specific panels have you > successfully used to detect anaplastic large cell lymphoma by flow > cytometry (we are experienced with ipox on sections)? I am waiting for > enlightenment so please share your pearls of wisdom. > > Maryalice > Maryalice Stetler-Stevenson > Director Flow Cytometry Unit > Laboratory of Pathology, NCI, NIH
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