-----Original Message----- From: Donnenberg, Albert Sent: Thursday, December 30, 1999 12:25 PM To: cyto-inbox Subject: RE: I got them compensation blues... Lest anyone draw the wrong conclusion, setting your compensation depends very much on the brightness of the compensation standards! You can only count on your compensation being correct for events as bright or dimmer than the standards. Mario wrote the book on this, but his statement might be misconstrued. Moral: If you use compensation standards at all (as opposed to setting compensation for each individual pair of reagents) the standards should be as bright as your brightest reagent. Happy Y2K. Albert Albert D. Donnenberg, Ph.D. Interim Director Bone Marrow Transplant Program, UPCI Phone: (412) 624-9596 Fax: (412) 624-9624 By the way, I want to remind everyone that compensation settings DO NOT DEPEND on the brightness of a reagent! Therefore, you should always use the brightest reagent you have in any given channel to set compensation!
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