Dear Rick, Why dont you try using the MACS system. http://www.miltenyibiotec.com/index.php?site=products-human Using anti-CD45 magnetic MicroBeads (for leucocytes) you might separate all cells of hematopoietic origin except erythrocytes, platelets and their precursor cells. Yours, Stoyan --- Rick Arthur Bright <rbright@emory.edu> wrote: > > I am trying to analyze lymphocytes, monocytes, > granulocytes, etc from > brain and lung tissues. After collagenase digestion > I, of course, have a > lot of junk...stuff I don't want to run through the > instrument. > > What is the best way of separating the cells from > the junk? I tried > Ficoll-paque, which nets me the lymphocytes, but I > lose other cells types > that might be important. > > Would a Percoll gradient be a better route to go? > What gradients would be > most appropriate. I am digging in the literature as > well, finding many > reference to percoll gradients, but few of them say > what the gradients are > and where the cells of interest will settle. > > Any quick thoughts would be helpful. I, hopefully, > will stumble upon that > magic reference sometime tonight, but if you know > already I would > appreciate your help. > > Thanks > > Rick > > > __________________________________________________ Do You Yahoo!? Yahoo! Tax Center - online filing with TurboTax http://http://taxes.yahoo.com/
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