I am trying to analyze lymphocytes, monocytes, granulocytes, etc from brain and lung tissues. After collagenase digestion I, of course, have a lot of junk...stuff I don't want to run through the instrument. What is the best way of separating the cells from the junk? I tried Ficoll-paque, which nets me the lymphocytes, but I lose other cells types that might be important. Would a Percoll gradient be a better route to go? What gradients would be most appropriate. I am digging in the literature as well, finding many reference to percoll gradients, but few of them say what the gradients are and where the cells of interest will settle. Any quick thoughts would be helpful. I, hopefully, will stumble upon that magic reference sometime tonight, but if you know already I would appreciate your help. Thanks Rick
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