A colleague asked a simple question regarding a continous lymphoblast system exposed to toxicants. What is the best assay or kit to detect apoptosis? After a short discussion on flow assays that included --permeability endpoints, annexin, tunnel, and subg1, he decided on using the gold standard of laddering. He wants a definitive test. I thought there were more sensitive and more accurate flow cytometic assays to detect apoptosis. I also told him he usually needs two tests to make a conclusion reegarding apoptosis. Any opinions on what is the best and easiest approach to detect apoptosis in a suspension cell culture? Bob Robert M. Zucker, PhD U.S. Environmental Protection Agency Office of Research and Development National Health and Environmental Effects Research Laboratory Reproductive Toxicology Division, MD 72 Research Triangle Park, North Carolina, 27711 Tel: 919-541-1585; fax 919-541-4017 e-mail: zucker.robert@epa.gov
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