Has anyone have experience on running a side population analysis of human bone marrow with the BD LSR? I'm using the Hoecst 33342 dye and a FITC cell surface marker and following the published protocol to the letter, but still cannot see any Hoechst fluorescence on the red channel. Filter setup? Software compensation? Any help would be much appreciated. Kimmo Porkka ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Kimmo Porkka, M.D., Ph.D. Helsinki University Central Hospital Dept. of Medicine, Division of Hematology P.O. Box 340, 00029 HUS Helsinki, Finland kimmo.porkka@hus.fi +358-9-471-951029 (HUCH) +358-9-471-71890 (Biomedicum) +358-400-492-100 (mobile) +358-9-471-71897 (fax) +1-810-283-8318 (eFax)
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:59:26 EST