One of the best known highly specific marker for human endothelial cells is CD146, also lnown as MUC18, MCAM or S-endo. BioCytex (specialized in whole blood quantitative flow cytometry) has developed 5 mouse monoclonal antibodies raised against CD146. All of these MAbs are commercially available. Dynal (www.dynal.no) is recommending to use anti-CD146 coupled to Dynabeads for enrichment of circulating endothelial cells from peripheral blood. Unfortunatly, such dynabeads are incompatible with flow cytometry. Please feel free to visit our web site (www.biocytex.fr) or to contact us directly if you have any need for additional information. Maxime Moulard, PhD BioCytex Research and Technology 140 Chemin de l'Armée d'Afrique 13010 Marseille France el.: 011 33 4 96 12 20 44 Fax: 011 33 4 91 47 24 71 Email: info@biocytex.fr Web site: www.biocytex.fr "Stetler-Stevenso, Maryalice (NCI)" <stetler@mail.nih.gov> le 15/02/2002 17:55:31 Pour : Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> cc : (ccc : MailingList/general/Biocytex) Objet : endothelial cells Does anyone have a method for measuring endothelial cells in the peripheral blood by flow cytometry? I understand this has been measured in sickle cell crisis. Maryalice Stetler-Stevenson, M.D., Ph.D. Chief, Flow Cytometry Unit Laboratory of Pathology, NCI, NIH Sometimes you're the windshield, sometimes you're the bug.
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