Wayne, I have not worked with PL cells but would suggest using either TcR-beta (H57-597) in PE, not FITC or a combination of CD4 and CD8 in the same color to identify a/b T-cells (PE or FITC OK). These work well with peripheral blood, LN and spleen cells. Sorry I can't help with a good g/d clone. -Jim Weaver ************************************************* * * * James L. Weaver Ph.D. * * Division of Applied Pharmacology Research * * Office of Testing & Research * * CDER MOD-1, FDA * * 8301 Muirkirk Rd, Laurel MD 20708 * * * * Phone: 301-827-8237 * * Fax: 301-594-3037 * * Email:WEAVER@CDER.FDA.GOV * * * * This email is my personal opinion and is NOT * * in any way official U.S. FDA Policy * ************************************************* * -----Original Message----- * From: Wayne A. C. Harris [mailto:waharri@emory.edu] * Sent: Tuesday, February 19, 2002 1:53 PM * To: Cytometry Mailing List * Subject: g/d t cell detection in mice * * * * Hello everyone, * * I just had a question. Wanted to know if anyone has a good * method for * murine g/d T cell detection in peritoneal lavage cells of B6 * and C3H mice. * I've simply been using g/d TCR PE (GL3-pharmingen) and CD3 PerCP * (145-2C11-pharmingen). Neither of these antibodies have * worked very well for * us individually(I'm not sure why?) and we have gone pretty * much on good * faith that together they help us pick out a population * (i.e.. cloud) we can * work with. The data seems quite 'noisy' and could be improved. * * If anyone has a better antibody or method. * * thanks, * * * -- * Wayne Harris * Lead Research Specialist * * Department of Microbiology and Immunology * Emory University , School of Medicine * * * * *
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