g/d t cell detection in mice

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Hello everyone,

I just had  a question. Wanted to know if anyone has a good method for
murine g/d T cell detection in peritoneal lavage cells of B6 and C3H mice.
I've simply been using g/d TCR PE (GL3-pharmingen) and CD3 PerCP
(145-2C11-pharmingen). Neither of these antibodies have worked very well for
us individually(I'm not sure why?) and we have gone pretty much on good
faith that together they help us pick out a population (i.e.. cloud) we can
work with. The data seems quite 'noisy' and could be improved.

If anyone has a better antibody or method.

thanks,


--
Wayne Harris
Lead Research Specialist

Department of Microbiology and Immunology
Emory University , School of Medicine

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