I would be concerned, in the technique described by Richard Haugland, that there would be free anti-fluorescein binding sites available to incorrectly bind the second fluorescein conjugated antibody. I have never used anti-fluorescein in this type of protocol, but I know from bitter experience that anti-immunoglobulins cannot be used in this way unless those free binding sites are blocked using excess immunoglobulin. I'm not saying it won't or can't work, just that one should be careful to confirm the specificity of the second reagent in such a protocol. Ken Ault
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:59:24 EST