Has anyone had any experience working with rat pancreatic islet cells? I have a client that wants to work with rat pancreatic islet cells. We want to discriminate between non B-cells and B-cells. We know that for example, Van der Wrinkel et al. established an elegant technique for sorting islet cells into discrete populations based on intrinsec cellular autofluorescence but we have some problems. For example: Is it necessary to incubate the cells in 2.8 mM of glucose at 17C for detecting autofluorescence of B-cells? How much time does incubation take? Do you know what happens with the autofluorescence of B-cells versus non B cells in cultures of 12-24 h with 11.1 mM of glucose at 37 C? Any information would be appreciated. Thanks in advance Esther Castaņo Campus de Bellvitge Universitat de Barcelona
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