Hi You can try do use a combination of CD3 and CD2. Many NK cells are CD3- and CD2+. About 10% of Cynomologous monkey are CD3- by using the FN18 clone for CD3. Best regards Lena Appelsved ___________________________________________________________ AstraZeneca R&D Södertälje, Local Discovery RA CNS/Pain Control Bioscience, Hälsovägen 7, S-141 57 Huddinge, Sweden Tel: +46 8 553 294 44 Fax: +46 8 553 25420 Lena.Appelsved@astrazeneca.com -----Original Message----- From: jill.omerza@pharma.novartis.com [mailto:jill.omerza@pharma.novartis.com] Sent: den 7 februari 2002 13:49 To: cyto-inbox Subject: NK Markers in Cynos Hi everyone, I was hoping to find someone who has some experience using markers to identify NK cells in Cynomologous Monkey peripheral blood. I have used a mixture of CD56+16 from Pharmingen (clones MY31 + 3G8) and after reading an article that states that CD56 may be identifying monos and not NK cells in Rhesus Monkeys, I have been doubting whether what I am measuring is just NK cells. Does anyone know if this is also true in Cynos as well? Are there any better markers available to measure NK cells, or is it sufficient to measure those cells that are just CD16 positive in this species. Any and all comments are appreciated. Jill Omerza Novartis Pharmaceutical Corp. 1 Health Plaza East Hanover, New Jersey 07936 973 781-8700
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