Fluorescein diacetate will stain live cells green, ethidium bromide will counterstain red for dead cells. For further details: Takasugi, M. An improved fluorochromatic cytotoxic test. Transplantation 1971 Aug 12(2):148-51 Ole J. Bergh, Supervisor Flow Cytometry Facility University of Pittsburgh Cancer Institute 200 Lothrop Street W 1009 Biomedical Science Tower Pittsburgh, PA 15213 Phone:412 624 0399 Fax: 412 624 9624 mailto:berghoj@msx.upmc.edu <mailto:berghoj@msx.upmc.edu> http://pci.upmc.edu/internet/flowcyto/flow.htm <http://pci.upmc.edu/internet/flowcyto/flow.htm> -----Original Message----- From: Michal Abel [mailto:abelm@necker.fr] Sent: Friday, February 01, 2002 3:43 PM To: cyto-inbox Subject: staining for viability I've got a question for the list regarding staining for viability. I am working with freesed/defrost lymphocytes. One of the drawbacks of this procedure is the number of death cells, which make higher the background noise. I wonder what kind of marker should I use for gating only the alive lymphocytes. Logically, I thought, the best would be necrosis marker like PI. However, I am not sure if I also need to eliminate the early apoptotic cells or not ? I would be grateful if someone could help me resolving this problem. Thanks, -- Michal Abel, MD, PhD Laboratory of Clinical Immunology INSERM U25 Necker Hospital, 161 Rue de Sèvres. Paris tel: 33 1 44 49 52 23 fax: 33 1 44 49 53 74
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