Dear cytometrists, I have a colleague who would like to look at DNA profiles of foetal cardiomyocytes by flow (MoFlo) using PI or EB. Andreas Simm had some problems a few years back with PI for the cardiomyocytes and I wondered if that had been solved satisfactorily whilst still allowing the cells to be kept intact for other markers? Also are there any protocols specifying the general flow conditions (nozzle size etc)? Many thanks, Bruce Milthorpe
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