Having spent many years conjugating many antibodies to phycobiliproteins, I can understand why you wouldn't want to do it yourself (although the results are ALWAYS better). For the last couple of years, however, we've been using Alexa633 instead, and I have to say that even using the straight-out-of-the-box protocol supplied by Molecular Probes, the results are quite good (if you have enough antibody to spare and want to test a couple of chrome:Ab ratios the results can be even better). I highly recommend giving it a try, it takes about one hour of hands-on time and about three hours in total. I should mention that Alexa660 works beautifully as well for flow, in fact better than 633 (I'd love to hear somebody at either MP or one of the equipment manufacturers explain why this should be the case; neither the optics nor the spectra would predict it). In fact after years as a dye laser loyalist, we now use HeNe exclusively, since we can simultaneously excite and detect A633 and A660 instead of APC and TR. Given the utter simplicity of conjugation of the Alexa dyes, it's worth considering. > To All: > > I am in need of getting two monoclonal antibodies conjugated to APC. > If you have had this done by some company/lab and liked the result >would you please let me know. > > thanks > > Jim Houston -- Howard T. Petrie, Ph.D. Head, Laboratory of Developmental Immunology Memorial Sloan-Kettering Cancer Center Box 341, 1275 York Avenue New York, NY 10021 phone (212)639-2149 fax (212)794-4019
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