Jáksó Pál <Pal.Jakso@aok.pte.hu> ha detto: > I get good results wihh CD10 Pe Caltag clone 5-1B4 or CD10 Fitc Coulter clone J5. See on purpose Am J Clin Pathol 2001;116:183-190 > Dear flowers, > > One of our pathologists complained about our CD10 flow results of lymph > node biopsies and fine needle aspirates, because they don't match with > immuohistochemical staining which shows positivity of lymph nodes > germinal center cells and follicular lymphoma while the flow shows > negativity or very dim stain although this antibody works well in B-cell > acut lymphoid leikaemia. Currently we use DAKO (clone: SS2/36) for flow > and Novocastra CD10 (clone: 56C6) for immunohistochemistry. I think the > different clones may give different staining. > Novocastra does not produce fluorescent labelled CD10 for flow and I > have to look for other sources to try different CD10 clones wich give > comparable result with the immunohistochemistry in the case of lymph > nodes. > Has anybody experience on this ? > > Thanks, > > Pal Jakso > > University of Pecs > Faculty of Medicine > Dept. of Pathology > 7643, Hungary > 12. Szigeti str. > --
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