Hi, We want to label CD8alpha expressing cells with an FITC conjugated Ab. This Ab works great on T cells (Pharmingen, clone 53-6.7). But we need to have our cells EtOH fixed for further DNA content analysis with PI, instead of the usual formaldehyde procedure, which is incompatible with PI DNA content analysis. Up to now, our first tests are not very promising. Does anybody out there have some experience on that point, and good advice? Thank you in advance! Philippe PS: by the way, if someone knows where we can get the 53-6.7 clone, I am sure our lab budget would appreciate the tip... ____________________________________________ Philippe Pognonec, Ph.D. Transcriptional Regulation and Differentiation Centre de Biochimie Parc Valrose Universite de Nice 06108 Nice cedex 2 France Tel/fax: (33) 492 07 64 13 ____________________________________________
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