Maris, I am quite sure that the background you are seeing in the APC channel is the red fluorescence of the hoechst. I am told that you may have a configuration problem with the MOFLO that can be rectified. I have spoken with Zip and Allen about this and they may be able to help. I know that I do not see this problem with the Vantage SE when I am running Argon(488nm), HeNe(633nm) and UV(350nm) lasers in separate time windows. David David M. Dombkowski dombkowski@helix.mgh.harvard.edu Flow Cytometry-Pathology-CNY rm7017 149 13th Street Massachusetts General Hospital-East Charlestown, MA 02129 Tel. (617)726-1683 Fax.(617)724-3164
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