are you saying it isnt the forum or it should not be ? big difference. there ARE lots of questions posted that could be answered by going to the literature. some people cant be bothered, but some people dont have access to the quick computer searches many of us take for granted, and sometimes the different opinions offered by list participants is more informative - or at least entertaining ;) than the literature. Rachel ======================================================= Rachel M. Gerstein, Ph.D. Department of Molecular Genetics and Microbiology Graduate Program in Immunology/Virology University of Massachusetts Medical School 55 Lake Avenue North Worcester, MA 01655-0002 (508) 856-1044 (508) 856-5920 (FAX) > ---------- > From: Paula Fukushima > Sent: Friday, December 14, 2001 9:35 AM > To: Gerstein, Rachel > Subject: RE: FACS question > > Hi Rachel, > That is true however this is not the forum where people do their > literature searchs. > > Paula > At 09:20 AM 12/13/2001 -0500, you wrote: > > >Aren't you being a bit harsh? > > > >I want to suggest that we all try to phrase our responses in a manner > that > >educates, but does not flog the person asking the question. Yes, its > really > >important to ask the right question, with a request for specific > >information. Yes, we all grind our teeth in response to some questions. > >Really poorly phrased questions tend not to get answered. However, there > >have been some very harsh responses in the last few weeks. We all were > new > >and/or ignorant at some point. Lets not scare away people who might > really > >benefit from the list ! > > > >just mho... > > > >Rachel > > > >======================================================= > >Rachel M. Gerstein, Ph.D. > >Department of Molecular Genetics and Microbiology > >Graduate Program in Immunology/Virology > >University of Massachusetts Medical School > >55 Lake Avenue North > >Worcester, MA 01655-0002 > >(508) 856-1044 > >(508) 856-5920 (FAX) > > > > > > > ---------- > > > From: J.Paul Robinson > > > Reply To: jpr@flowcyt.cyto.purdue.edu > > > Sent: Tuesday, December 11, 2001 9:20 PM > > > To: Cytometry Mailing List > > > Subject: Re: FACS question > > > > > > > > > Colleagues: I am going to answer this question in a manner that I hope > you > > > all > > > understand (or not)......(think of the bad data issue) > > > > > > You can solve the problem by adding reagent A to reagent B, plotting > FL3 > > > Vs FL2. > > > If you add reagent C, then a single histogram of FL5 should do the > trick. > > > I think you > > > could also try probe A and probe B, both of which should have the > right > > > spectra...Oh, > > > turn the power of laser 1 up a bit, and you should get some valuable > > > data..... > > > > > > sorry, I couldn't resist it... > > > please stop using terms which are totally undefined.... > > > > > > Paul Robinson > > > > > > > > > On 10 Dec 2001, at 11:58, Laura H > > > odges wrote: > > > > > > > > > I'd like to post a question on your website: > > > > > > > > > Can anyone suggest another P-gp accumulation probe > > > other than rhodamine 123 which has too broad of an > > > emission spectrum for my use. I am trying to find a > > > P-gp probe that can be used with other fluorescent > > > markers in channels FL2, FL3, and FL4 in a multicolor > > > assay. > > > > > > > > > __________________________________________________ > > > Do You Yahoo!? > > > Send your FREE holiday greetings online! > > > http://greetings.yahoo.com > > > J.Paul Robinson, PhD PH:(765)4940757 > > > Professor of Immunopharmacology > > > Professor of Biomedical Engineering > > > Purdue University FAX:(765)4940517 > > > EMAIL:jpr@flowcyt.cyto.purdue.edu > > > WEB: http://www.cyto.purdue.edu > > > > > > > > > > Paula Fukushima > Flow Cytometry > LP, NCI, NIH > 10 Center Drive MSC-1500 > Bethesda, Md. 20892-1500 > > > >
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