Jack I have one lab that has put DsRed2 in cells. They say it is brighter than DsRed1 (analysis done on FACSCalibur with standard filters - use PE channel) otherwise all I know is what Clonetech says about maturation rate, toxicity and aggregation. Larry At 05:42 PM 12/13/2001, you wrote: >Dear Larry: > >Our lab are very interested in the DsRed2 and needs some advice from >experts. >Do you have any new information about the DsReds? How is it going in your >lab? > >Thanks a lot and Best Wishes, > >Jack > >----- Original Message ----- >From: Larry Arnold <lwarma@med.unc.edu> >To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> >Sent: Wednesday, November 28, 2001 10:32 AM >Subject: Re: RFP > > > > > > You should be aware, if your'e not, that Clonetech now has DsRed2 which > > apparently solves many of the problems with DsRed (now called DsRed1) - > > faster maturation, low toxicity, low aggregation, brighter. We have > > several users working it up so I should know soon the results. > > > > Larry > > > > At 04:53 AM 11/28/2001, you wrote: > > > > >Dear Philippe, > > > > > >DsRed is a difficult beast to work with. Main problems are slow >maturation > > >and aggregation. We are submitting a paper on a tight link between > > >aggregation (minimum is a dimer) and generation of a functional > > >chromophore. I will be glad to share the results with you once we have > > >feedback from the journal. > > > > > >ciao > > > > > >Saverio > > > > > >Saverio Alberti > > >Head, Lab. of Experimental Oncology > > >Department of Cell Biology and Oncology > > >Consorzio Mario Negri Sud > > >66030 Santa Maria Imbaro (Chieti), Italy > > >Phone: (39-0872) 570.293 > > >FAX: (39-0872) 570.412 > > >E-mail: alberti@cmns.mnegri.it > > > > > > > > >On Mon, 26 Nov 2001, Philippe Pognonec wrote: > > > > > > > > > > > Dear all, > > > > to track our transfected cells, we have to use a red fluorescent > > > protein. We tried > > > > pDS Red N1 (Clontech), but the signal obtain on our FAcSort is really > > > bad (FL2 ), at > > > > least as compared to what we get when we use pEGFP N1 (FL1). > > > > Is anyone aware of a "better" red FP, that is efficiently excitable > > > with the argon > > > > laser, and displays a good and bright emission? > > > > Philippe > > > > ____________________________________________ > > > > > > > > Philippe Pognonec, Ph.D. > > > > Transcriptional Regulation and Differentiation > > > > Centre de Biochimie > > > > Parc Valrose > > > > Universite de Nice > > > > 06108 Nice cedex 2 > > > > France > > > > Tel/fax: (33) 492 07 64 13 > > > > http://www.unice.fr/biochimie/u470/prog5us.htm > > > > ____________________________________________ > > > > > > > > > > > > > > > > > > > > > > > > Larry W. Arnold, Ph.D. > > Associate Professor > > Director, Flow Cytometry Facility > > Department of Microbiology and Immunology > > Lineberger Comprehensive Cancer Center > > CB# 7290 > > University of North Carolina at Chapel Hill > > Chapel Hill, NC 27599 > > Phone: 919-966-1530 > > FAX: 919-962-8103 > > Larry W. Arnold, Ph.D. Associate Professor Director, Flow Cytometry Facility Department of Microbiology and Immunology Lineberger Comprehensive Cancer Center CB# 7290 University of North Carolina at Chapel Hill Chapel Hill, NC 27599 Phone: 919-966-1530 FAX: 919-962-8103
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