You should be aware, if your'e not, that Clonetech now has DsRed2 which apparently solves many of the problems with DsRed (now called DsRed1) - faster maturation, low toxicity, low aggregation, brighter. We have several users working it up so I should know soon the results. Larry At 04:53 AM 11/28/2001, you wrote: >Dear Philippe, > >DsRed is a difficult beast to work with. Main problems are slow maturation >and aggregation. We are submitting a paper on a tight link between >aggregation (minimum is a dimer) and generation of a functional >chromophore. I will be glad to share the results with you once we have >feedback from the journal. > >ciao > >Saverio > >Saverio Alberti >Head, Lab. of Experimental Oncology >Department of Cell Biology and Oncology >Consorzio Mario Negri Sud >66030 Santa Maria Imbaro (Chieti), Italy >Phone: (39-0872) 570.293 >FAX: (39-0872) 570.412 >E-mail: alberti@cmns.mnegri.it > > >On Mon, 26 Nov 2001, Philippe Pognonec wrote: > > > > > Dear all, > > to track our transfected cells, we have to use a red fluorescent > protein. We tried > > pDS Red N1 (Clontech), but the signal obtain on our FAcSort is really > bad (FL2 ), at > > least as compared to what we get when we use pEGFP N1 (FL1). > > Is anyone aware of a "better" red FP, that is efficiently excitable > with the argon > > laser, and displays a good and bright emission? > > Philippe > > ____________________________________________ > > > > Philippe Pognonec, Ph.D. > > Transcriptional Regulation and Differentiation > > Centre de Biochimie > > Parc Valrose > > Universite de Nice > > 06108 Nice cedex 2 > > France > > Tel/fax: (33) 492 07 64 13 > > http://www.unice.fr/biochimie/u470/prog5us.htm > > ____________________________________________ > > > > > > > > > > Larry W. Arnold, Ph.D. Associate Professor Director, Flow Cytometry Facility Department of Microbiology and Immunology Lineberger Comprehensive Cancer Center CB# 7290 University of North Carolina at Chapel Hill Chapel Hill, NC 27599 Phone: 919-966-1530 FAX: 919-962-8103
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