The best way to do this with a small sample is by PCR. Our tissue typing lab does not use flow for typing. There are several commercially available kits. I suggest that you speak with your tissue typing lab to find out which they use Martin Zand University of Rochester Medical Center Rochester, NY -----Original Message----- From: Frank Mattes [mailto:list_server2001@yahoo.com] Sent: Friday, November 23, 2001 12:22 PM To: cyto-inbox Subject: help HLA-I typing Dear FACS expert, to begin, I'm just a beginner in the FACS world, so I hope I'm not upsetting anyone with my question. For my research project, I need to know the HLA-I type of the patients under study. I thought the quickest way would be to HLA type on a aliquot of the bood sample I received. I don't need the full HLA-type, I need only to know if the patient is A2, B7, B8, B35. I'm wondering if someone know a method to do that on a small blood volume ( 100 micol)without isolating PBMCs. My 2nd question is, if the Ab are comercially available. I appreciate your help Frank Mattes Department of Virology Royal Free Hospital, London __________________________________________________ Do You Yahoo!? Yahoo! GeoCities - quick and easy web site hosting, just $8.95/month. http://geocities.yahoo.com/ps/info1
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