help HLA-I typing

From: Frank Mattes (list_server2001@yahoo.com)
Date: Fri Nov 23 2001 - 12:21:38 EST


Dear FACS expert,

to begin, I'm just a beginner in the FACS world, so I
hope I'm not upsetting anyone with my question.

For my research project, I need to know the HLA-I type

of the patients under study. I thought the quickest
way would be to HLA type on a aliquot of the bood
sample I received.
I don't need the full HLA-type, I need only to know if
the patient is A2, B7, B8, B35.
I'm wondering if someone know a method to do that on a
small blood volume ( 100 micol)without isolating
PBMCs.

My 2nd question is, if the Ab are comercially
available.

I appreciate your help

Frank Mattes
Department of Virology
Royal Free Hospital, London

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