Re: Bad FACS data presentation -- more discussion points.

From: Howard Shapiro (hms@shapirolab.com)
Date: Tue Nov 06 2001 - 21:52:48 EST


Ann Atzberger wrote-

>if the "odd" scientist can get inappropriate data published; then
>personally I believe that is his God given right.

Well, we (the flow community) know there is a lot of bad flow in a lot of
good journals, and Mario intimated that flow might not be the only
technical area in which there are problems.  I am not well versed in the
details of gel electrophoresis, the blots in various directions of the
compass, and gene arrays, to name a few, but there certainly is a lot of
data acquired using those technologies in the current literature.  Does
anybody in our gang know any gel curmudgeons, blot curmudgeons, and/or
array curmudgeons (maybe arrays are too new for there to be any of these)
who rant about how crappy the gel, blot, and array data are in the
published literature? Is bad flow just the tip of the iceberg? (I haven't
mentioned confocal because I know there are confocal curmudgeons, at least
some of whom are readers of the Cytometry Mailing List).

Let's face it.  A paper is reviewed by two or three people, and they can't
know everything about everything.  And, these days, it's very unlikely that
a paper with ten or more authors will include data obtained using only one
or two of the methods mentioned above.  I have to admit that I am a benign
reviewer, and I tend to give authors the benefit of the doubt about a lot
of their data, and even to refrain from complaining about flow data
displayed in a manner that I don't think is up to my highest
standard.  When I function as an editor or associate editor, I try to send
a paper I will review to somebody else who is likely to know the details in
areas where I feel least secure, so that, between us, we cover as many
bases as possible.  But we'd need a half-dozen reviewers to make sure that
all the data passed muster, and I don't see that happening.

Most of the time, the bad flow data doesn't invalidate the principal
conclusion(s) of a paper. When it does, the best way to deal with it is to
produce another paper using good flow data to reach the right
conclusion.  And the same is true for all of the other technologies. When
somebody publishes conclusions that are unjustified or flat out wrong,
setting the record straight isn't defamation or libel, and the
repercussions may be just what is needed to get people to pay more
attention to their data.

And an aside - if we agree that people shouldn't label axes "FL1", "FL2",
etc., isn't it about time we got them to substitute "flow cytometry data"
for "FACS data"? All FACSes are flow cytometers, but not all flow
cytometers are FACSes...and some FACSes, such as FACScans and FACSCounts,
aren't even Fluorescence Activated Cell Sorters.

-Howard



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