Hi Geoff, Quick question. Your histogram is encouraging. To clarify, the CD8 is a fluorochrome other than the Alexa350 and the CD45.1 is the Alexa350? When will you have the "optimal" set-up to let the rest of us with LSRs know if it works? Randy T. Fischer NIH/NIAMS Building 10, Room 6D57 9000 Rockville Pike Bethesda, MD 20892 (301) 594-3537 fischer1@mail.nih.gov > ---------- > From: Geoffrey Osborne > Sent: Monday, September 10, 2001 10:03 PM > To: Cytometry Mailing List > Subject: Re: BD LSR UV LASER > > > Hi Eugene, > Last week we were testing Alexa350 on the LSR. I've just whipped up > a web > page showing some preliminary results. > http://jcsmr.anu.edu.au/facslab/LSRexample.html > > Check it out. It's not brilliant but under certain conditions/caveats it > should be useful. > > Geoff > > At 10:34 PM 9/9/01 -0400, Pizzo,Eugene wrote: > > > >Hi folks, > > > >If anyone has tried using the BD LSR to visualize a UV excitable > >fluorochrome > >for cell surface staining, I wonder if they might comment on their > results. > >I'm aware it isn't generally possible, that it's best suited to cell > cycle > >and calcium flux but what about limited use of AMCA or Cascade Blue > >for antigens having very strong signals? > > > >Gene/UCONN Health > > > > > Geoffrey Osborne > > Specialist, Flow Cytometry, > John Curtin School of Medical Research, > The Australian National University, > Canberra, 0200, ACT. AUSTRALIA > email: geoff.osborne@anu.edu.au > http://jcsmr.anu.edu.au/facslab/facshome.html > > (61 2) 6125 3694. > >
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