Tami, Are you sure your autofluorescence is caused by the lipofectamine? Don't you normally wash off the DNA/lipofectamine complex at least 24 hours before analysis? If not I suggest you do and replace with fresh medium, then leave your cells at least 24/48 hours before analysis. The other thing you might try is resuspending your cells in a phenol-red free medium just prior to analysis. Phenol red can cause autofluorescence. I hope that's helpful. Kind regards Simon trosario@cellmate.cb.uga.edu on 31-Aug-2001 21:52 To: cyto-inbox cc: Subject: Lipofectamine auto fluorescence We are transfecting COS 7 cells with a plasmid containing GFP using lipofectamine. We see a large amount of auto fluorescence from the lipofectamine alone in our controls. Has anyone else experienced this? If so, how can we remedy the situation. Thanks for your help, Tami Rosario Department of Cellular Biology University of Georgia
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:57:52 EST