Greetings all, I am acquiring a I-90 laser to go alongside the HeNe and air-cooled Ar units on our Elite. I routinely use FITC/PE/PerCP/APC (APC time gated on PMT4) in our current configuration and would like to preserve this with the possibility of adding AMCA/Cascade blue as an extra colour oh, and i'd like also to be able to use the 514 line! -I guess my question is; which arrangement of laser optics i.e. beamsplitters, mirrors etc. is lilkely to give me the greatest flexibility. I apreciate this might be an impossible question to answer: too many variables, but I think it would be informative to hear how others have configured similar systems (multi-line Ar, 488nM Ar, HeNe) Thank you in advance Arnold _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/ Arnold Richard Pizzey Department of Haematology Royal Free and University College London Medical School 98 Chenies Mews London WC1E 6HX U.K voice: +44 020-7679-6234 Fax: +44 020-7679-6222 email: a.pizzey@ucl.ac.uk _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/
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