Hi Tami, Have you tried compensating (on a plot of fl2/fl1) between the lipofectamine fluorescence and GFP fluorescence? We do this pretty routinely so that we can separate low expressing transfectants from highly auto-fluorescent non-transfectants. I'm not sure that it'll work for lipofectamine, but it's easy to try. Cheers, Ray > From: Tami Rosario <trosario@cellmate.cb.uga.edu> > Date: Fri, 31 Aug 2001 16:52:05 -0400 > To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> > Subject: Lipofectamine auto fluorescence > > > We are transfecting COS 7 cells with a plasmid containing GFP using > lipofectamine. We see a large amount of auto fluorescence from the > lipofectamine alone in our controls. Has anyone else experienced > this? If so, how can we remedy the situation. > Thanks for your help, > Tami Rosario > Department of Cellular Biology > University of Georgia > >
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