Greetings Ole, As far as I am aware, Hanks Buffered Saline Solution should be pH 7.4 If the stuff you are using isn't, try this recipe: 0.40 g KCl (5.4 mM) 0.09 g Na2HPO4x7H2O (0.3 mM) 0.06 g KH2PO4 (0.4 mM) 0.35 g NaHCO3 (4.2 mM) 0.14 g CaCl2 (1.3 mM) 0.10 g MgCl2x6H2O (0.5 mM) 0.10 g MgSO4x7H2O (0.6 mM) 8.0 g NaCl (137 mM) (1.0 g D-glucose (5.6 mM)) I would be a bit wary of including the Glucose myself; if your cells absolutely need glucose I would add it into the receiving tubes -or add glucose sequentialy through the sort. Adjust pH to 7.4 and add H2O to 1 liter and Filter sterilize Store indefinitely at 4°C If you find HBSS better for viability, let us know. Regards, Arnold At 03:10 PM 8/24/01 -0400, you wrote: > Sheath fluid for cell sorting Recently I was told that HBSS is >preferable to regular PBS based sheath fluids. It is supposed to increase >viability in sorted cells. Our problem is that HBSS is buffered with >Na-Bicarbonate, it also contains glucose. If we made up a 10-liter batch >and let it sit, it would turn alkaline. With the glucose as a nutrient >there is also an increased risk of growth. We would like to know what the >general opinion is on this. Ole J. Bergh, Supervisor Flow Cytometry >Facility University of Pittsburgh Cancer Institute 200 Lothrop Street W >1009 Biomedical Science Tower Pittsburgh, PA 15213 Phone:412 624 0399 >Fax: 412 624 9624 mailto:berghoj@msx.upmc.edu >http://pci.upmc.edu/internet/flowcyto/flow.htm _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/ Arnold Richard Pizzey Department of Haematology Royal Free and University College London Medical School 98 Chenies Mews London WC1E 6HX U.K voice: +44 020-7679-6234 Fax: +44 020-7679-6222 email: a.pizzey@ucl.ac.uk _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/
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