Hello Ole, The way that I handle the sheath fluid situation is to request that all of the investigators bring their own sheath fluid for the sort.... this solves all the problems with questionable osmolarity and contamination and I tell them to bring the solution where the cells are happiest ..... one word of caution.... I recommend to them to not use any fetal calf..(gets stringy and plugs the machine) or BSA...(can get foamy) in the sheath........On the collection side tubes... they can have straight fetal calf if they want....just another note.... on the table top analysis machines.. I use plain water.... by the time the cells mix with the hypotonic solution.. they have already been read and in the waste container.... saves you some money and doesn't salt up the machine as much.... I hope this helps.. Jim Phillips University of Miami School of Medicine PS.... check out my music CD at <http://www.mp3.com/jim_phillips> www.mp3.com/jim_phillips -----Original Message----- From: Bergh, Ole [mailto:berghoj@MSX.UPMC.EDU] Sent: Friday, August 24, 2001 2:10 PM To: cyto-inbox Subject: Sheath fluid Sheath fluid for cell sorting Recently I was told that HBSS is preferable to regular PBS based sheath fluids. It is supposed to increase viability in sorted cells. Our problem is that HBSS is buffered with Na-Bicarbonate, it also contains glucose. If we made up a 10-liter batch and let it sit, it would turn alkaline. With the glucose as a nutrient there is also an increased risk of growth. We would like to know what the general opinion is on this. Ole J. Bergh, Supervisor Flow Cytometry Facility University of Pittsburgh Cancer Institute 200 Lothrop Street W 1009 Biomedical Science Tower Pittsburgh, PA 15213 Phone:412 624 0399 Fax: 412 624 9624 <mailto:berghoj@msx.upmc.edu> mailto:berghoj@msx.upmc.edu <http://pci.upmc.edu/internet/flowcyto/flow.htm> http://pci.upmc.edu/internet/flowcyto/flow.htm <http://pci.upmc.edu/internet/flowcyto/flow.htm>
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