We use use Rhodamine, that uses the same channel as PE. On Sun, 12 Aug 2001, Ronald Rabin wrote: > > Jim, you cannot use PE on a fluorescent scope. I know this is true for > confocal, and am pretty sure it is true for any fluorescent microscopy. It > bleaches too quickly. Three colors you can use are FITC, Cy3 and Cy5. Pay > attention to the filter cubes, because there is spectral overlap between Cy3 > and FITC on one end, and Cy5 on the other. Also, you have too have a camera > and imaging system to "see" Cy5, as it is barely visible with the naked eye. > > ron > > > on 8/10/01 12:33 PM, jim phillips at jphillip@med.miami.edu wrote: > > > > > Hello to everyone, > > This is a little off the subject of flow... but I have an investigator > > that want to do 3 color cell surface fluorescense on a microscope. The > > question is .. what three fluorochomes can she use.. The microscope is > > just a normal fluorescense scope. I know FITC and PE but is there a > > third?? Can you use Cychrome??? She wants to view all three at the > > same time.. any help would be appreciated.. Thanks... > > Jim Phillips > > University of Miami School of Medicine. > > > > ps.... I just finished my music CD .. entitled ... "222.... 2 old? 2 > > fat? 2 ugly?" You can listen to it on mp3.. > > > > www.mp3.com/jim_phillips > > > > Ronald L. Rabin, M.D. > Senior Staff Fellow > Laboratory of Immunobiochemistry > DBPAP/OVRR > Center for Biologics Evaluation and Research > U.S. Food and Drug Administration > 29 Lincoln Drive (MSC-4555) > Building 29, Room 129 > Bethesda, MD 20892-4555 > > phone: 301.496.8806 > fax: 301.402.5177 > email: rr84g@nih.gov > >
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