Arnold CyChrome from Pharmingen is my preferred source of this reagent. Alternatively to use the 633 line try Cy5 or Alexa647 (we have tried the later and it works well). Larry At 05:52 AM 7/10/2001, you wrote: >Greetings all, > >Over the last few years I have been assaying G-CSF receptors using >biotinylated ligand on various cell types. By far the best second layer for >maost cell types has been streptavidin-RED670 from Gibco/BRL, sadly due to >the invitrogen merger, many lines have been discontinued -one of them being >my second layer of choice. > >I wonder if any others have had some experience of alternate second layers; >the excitation may be either 488 or 633. > > >Regards, > > >Arnold > >_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/ > Arnold Richard Pizzey > Department of Haematology > Royal Free and University College London Medical School > 98 Chenies Mews > London WC1E 6HX > U.K > > voice: +44 020-7679-6234 > Fax: +44 020-7679-6222 > email: a.pizzey@ucl.ac.uk >_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/ Larry W. Arnold, Ph.D. Res. Assoc. Prof. Director, Flow Cytometry Facility Department of Microbiology and Immunology Lineberger Comprehensive Cancer Center CB# 7290 University of North Carolina at Chapel Hill Chapel Hill, NC 27599 Phone: 919-966-1530 FAX: 919-962-8103
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