Greetings all, Over the last few years I have been assaying G-CSF receptors using biotinylated ligand on various cell types. By far the best second layer for maost cell types has been streptavidin-RED670 from Gibco/BRL, sadly due to the invitrogen merger, many lines have been discontinued -one of them being my second layer of choice. I wonder if any others have had some experience of alternate second layers; the excitation may be either 488 or 633. Regards, Arnold _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/ Arnold Richard Pizzey Department of Haematology Royal Free and University College London Medical School 98 Chenies Mews London WC1E 6HX U.K voice: +44 020-7679-6234 Fax: +44 020-7679-6222 email: a.pizzey@ucl.ac.uk _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/
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