Just caught your comments about BD's trucount beads. They can now be purchased separately, without any mAb included. They have unofficially been available through your sales rep for quite a while, but can now be order for about $305 for 50 (roughly) tubes and have an official part number from BD. They are not discounted at all yet, like the sheath fluid, since it is a "new" product. Still, it is cheap for the accurate count and QC performance. Check it out. Good Luck, Rick A. Bright Emory University Centers for Disease Control Influenza Branch -----Original Message----- From: Mark Kukuruga [mailto:kukuru@med.umich.edu] Sent: Sunday, June 17, 2001 1:20 PM To: cyto-inbox Subject: Re: absolute cell counts using FACS Karim, Long before we had people making counting beads for us, people were doing this themselves with any bead they had that was easily detected separate from cells either by 1) size, and/or 2) fluorescence. Products available today are advantageous from a QC angle, since they've been "optimized" for single suspension, and they've been assayed for count and fluorescence. These are very useful . . . if you think it's worth the added cost. I think in many cases, it is, since one can spend equally (or more) simply getting something to work. But, you could simply count some beads you already have, add a known number to a known volume, then do your own volume calibration. If this is any indication . . . A recent query suggested Coulter's Flow Count beads - - we're trying those. MAK. >>> Karim Vermaelen <Karim.Vermaelen@rug.ac.be> 06/15/01 17:11 PM >>> x-mac-creator="4D4F5353" Content-Transfer-Encoding: 7bit Hi everybody , Can anyone recommend a microsphere kit for performing simple cell counts on numerous samples using the FACS? BD's Truecount beads are only sold along with a MAb. Our cells do not have to be labelled. Any simpe alternative? (NB: our good ol' Coulter particle counter is dead) Tx Karim
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