As a follow-up to Jason's input... Does anyone have any really impressive numbers? Such as 50K-70K events (cells?)/sec, what kind of "events" or cells, recovery, etc. I know you can predict recovery, loss, and whatever else you can apply a statistic to, but I'm more for the actual tales! I'm just kind of curious, 'cause I've had no need to go below 70 micron nozzle-size, which at ~100KHz would allow for 20K-25K cells/sec, if we follow the 4-5 drops/event idea. I've gone "faster", i.e. 35K cells/sec, but didn't verify actual loss. I'd like to hear from someone sorting with a trigger rate of 70K/sec; I know the companies make certain claims, and once this information becomes mainstream, people don't want to hear that the rate only "works" for sperm or chromosomes or bacteria. They come to you with some 25 micron cells, and expect you to get 99% purity with little or no loss...all at the claimed 70K/sec. As flow operators/facilities, we deal with them, but it's frustrating! Anybody who replies can feel free to direct the mail to me, and if there's interest, I'll post the summary to the list. Andrew (who's pretty darned pleased with the MoFlo!) -----Original Message----- From: JASON MCCORMICK [mailto:jason.mccormick@regpha.com] Sent: Monday, May 21, 2001 8:38 AM To: cyto-inbox Subject: Re- Digital Flow Electronic Importance: Low While such advertising may be of questionable integrity on Cytomation's part, isn't it about time someone slung a little mud in the other direction? I have had sales reps, from a certain company, lie right to my face about the capabilities of their instruments and the limitations of MoFlo. IMHO, it seems more lacking in integrity and intelligence to tell me that my MoFlo cannot sort >20,000 cells/sec, because that violates the laws of physics. Especially lacking when one considers that I ROUTINELY sort at >30,000 cells/sec with >95% purity and >75% recovery (on a variety of cell types). I'm not the only one verifying these numbers.... Anyhow, I don't mean to flame anyone. I just thought this needed said. As far as digital electronics go, I'm no expert. Mario is probably right that DiVa and MoFlo electronics perform similarly (and he can test this). I guess I just don't feel comfortable always doing impossible sorts!
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:57:39 EST