Dr. She, Another instance of cells that are apoptotic and Annexin V negative is reported in J. Histochem. Cytochem. 46, 895 (1998) Comparison of Annexin V and Calcein AM as early Vital Markers of Apoptosis in Adherent Cells by Confocal Laser Microscopy. The authors attempted to correlate Annexin V-FITC binding (using NIH 3T3 cells) with changes in morphology. They found a subpopulation of cells that were apoptotic, but did not bind Annexin V. To quote: "...some cells that clearly exhibited apoptotic changes on calcein visualization were annexin-negative." Just another example of apoptosis +, Annexin V - Michael Kuhn Helix Research Fluorescence Chemistry mkuhn@helixresearch.com www.helixresearch.com
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