before I summarize my last question about the stimulation of monocytes and measurement of intracellular interleukins, I want to add one additional question: what is the reason to add serum to the basal medium (for example RPMI) even if one stimulate whole blood. In this case, one dilute blood 1:1 or up to 1:5 with medium. But even in the case of 1:5, there is still 20% plasma in the background. Andreas Andreas Simm Klinik fuer Herz- und Thoraxchirurgie Universitaet Halle Ernst-Grube-Str. 40 06120 Halle Tel.: +49 (0) 345 557 2647 FAX: +49 (0) 345 557 2782
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