I have been able to see Alexa350 on a BD LSR running about 15mw on He-Cad laser. I also routinely run dye on a sorter with 100-150mw UV laser. I find that dye is quite readily excited with 325 or 350 UV laser and easily detected. I recommend a 450BP40 filter to detect emmision. David M. Dombkowski dombkowski@helix.mgh.harvard.edu Flow Cytometry-Pathology-CNY rm7017 149 13th Street Massachusetts General Hospital-East Boston, MA 02129 Tel. (617)726-1683 Fax.(617)724-3164
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:57:37 EST