Hi James I did quite a bit of rat chromosome sorting a few years back. We published some stuff including flow karyotype of male Rattus Norvegicus in : Isolation of rat chromosome specific paint probes by bivariate flow sorting followed by degenerate oligonucleotide primed PCR. 1994 B. Hoebee, J. M. de Stoppelaar, R. F. Suijkerbuik and S. Monard Cytogenetics and Cell Genetics 66 pp277-282 The y chromosome is pretty easy to isolate. We used the same chromosome isolation protocol as we used with human. The procedure we used is described in detail in Methods in Molecular Biology Vol 91 where there is also a picture of a normal male rat flow karyotype. Good luck, let me know if you have any problems. Simon Monard FACS Lab Manager Trudeau Institute Saranac Lake NY12983 Ph 518 891 3080 X352 >>> "james.clark7" <james.clark7@ntlworld.com> - 4/17/2001 11:48 AM >>> Hi, I was planning to try and sort the rat Y chromosome. However in the literature there is only the mention of mouse and human Y sorting. Has anyone in the list attempted sorting either mouse or human Y chromosomes and could they give me some advice on the best way to attempt this in the rat? Best wishes James
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