Just a quick questions. I have had trouble phrasing the search properly of the e-mail discussions, but there was a discussion a few years ago concerning high backgrounds for PE-Cy5 staining. (The samples are coming up positive with Isotype staining - I know, Isotype staining might not be a true negative, but that is a different discussion. :-)) Is there a solution for the high background other than using some other fluorochrome like PerCp which doesn't have this problem, as I understand it. Thank you in advance for your help. Doug {-----------------------------------------------------------------------} Douglas Smoot NIDDK-Navy Transplantation & Autoimmunity Branch Naval Medical Research Center AFRRI Building 46, Room 2415 8901 Wisconsin Avenue Bethesda, MD 20889-5603 voice: 301-295-1843 fax: 301-295-6484
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