PE-Cy5 Backgrounds

From: Douglas S. Smoot (rin0dss@bumed30.med.navy.mil)
Date: Thu Apr 12 2001 - 15:10:03 EST


Just a quick questions.  I have had trouble phrasing the search properly of
the e-mail discussions, but there was a discussion a few years ago
concerning high backgrounds for PE-Cy5 staining.  (The samples are coming
up positive with Isotype staining - I know, Isotype staining might not be a
true negative, but that is a different discussion. :-)) Is there a solution
for the high background other than using some other fluorochrome like PerCp
which doesn't have this problem, as I understand it.  Thank you in advance
for your help.

Doug
{-----------------------------------------------------------------------}
Douglas Smoot
NIDDK-Navy Transplantation & Autoimmunity Branch
Naval Medical Research Center
AFRRI Building 46, Room 2415
8901 Wisconsin Avenue
Bethesda, MD 20889-5603
voice: 301-295-1843
fax: 301-295-6484



This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:57:33 EST