I am sending this again because the replies I have gotten are all talking about LARGE particles. These are tiny, little 100 nanometer particles. Now, are there any suggestions? What I sent originally: One of my reseachers is having difficulty separating a mixture of glass spheres and glass-coated, colloidal Au, core-shell particles. The free glass spheres nucleate and grow under the reaction conditions for the growth of the core-shell particle. The typical ratio of the free glass spheres to core-shell structures is ~100:1. The size of the core-shell particles is between 100-115 nm in diameter. The free silica particles are only slightly smaller in diameter (by about 5-10 nm). He has tried gradient separation and gradient centrifugation with a high MW dextran and low MW PEG, but has not come across appropriate conditions for separation. He is currently exploring the use of flow cytometry. Any suggestions are greatly appreciated? **************************************************************************** Elaine Kunze Flow Cytometry.....Image Analysis... Life Sciences Consortium 8B Althouse Laboratory (814-863-2762) Penn State University University Park, PA 16802
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