Doug, There is a paper Cytometry 37:41-50 (1999) by Janice Clement's group that looks at the identification of NK cells in rhesus. They report that 95% of CD16+ cells co-express CD8. They report that CD16+CD8+ cells in macaques are NK cells & that CD16+CD8+CD3+ cells are NK-T cells. They also report that CD56 identifies monocytes in rhesus PBMC. We have also seen the majority of CD16+ lymphocytes co-expressing CD8 in cyno PBMC. I hope this helps you. denise --On Fri, Mar 16, 2001 8:40 AM -0500 "Reed, Doug S Dr USAMRIID" <Doug.Reed@DET.AMEDD.ARMY.MIL> wrote:r > > Jan, > > My inclination would be say #3. We have just recently come upon what I > would describe as a fairly unusual situation in which we see CD16 > expression on CD8lo cells in macaque PBMCs (from virally infected > animals, by > coincidence). These cells have all the classical "signs" of activated > cells - increased expression of CD44 & CD95, increased cell size, and > granzyme B+. Our original decision to go with CD16 was based on the > description that it was expressed on monocytes and not T lymphocytes - > but now that appears not to be the case (at least with macaques). > > Has anyone else seen CD16 expression on T lymphocytes? > > -Doug Reed > > -----Original Message----- > From: Muellerberghaus, Jan [mailto:muellerberghausj@MSX.UPMC.EDU] > Sent: Monday, March 12, 2001 5:47 PM > To: cyto-inbox > Subject: NK, NK-T, T cell or what? > > > > Dear flowers: > > I am cloning human T cells specific for viral antigens and have slightly > confusing result now when I phenotype the clones. They are all CD3+, all > CD8+, all CD4-, all HLA-DR+ and now comes the confusing part 10-30% > positive for CD56 and 5-10% CD16 positive (the last stimulation with > irrradiated feeders and PHA is 12 days ago, the cells are cultured in > IL-2 at 1000 U/ml). > > What are these cells: 1) remaining feeders? 2) my cultures are not clonal? > 3) in vitro phenomenon because of culture condition and stimulation? 4) > NKT cells? > > Help with resolving this is appreciated > Cheers > Jan > Denise A. Croix, Ph.D. Research Associate Dept of Molecular Genetics & Biochemistry, W1111 BST Univ. of Pittsburgh School of Medicine Pittsburgh, PA 15261 412-624-8664 (work) 412-648-3394 (fax) 412-958-1646 (pager)
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