Assuming your feeders are gone, #3. Cultured, stimulated lymphocytes can pick up these NK associated markers. You will likely find a small % [approx 3%] of CD56+ CD8+3+ in normal circulating cells, too. F Preffer At 05:46 PM 3/12/01 -0500, Muellerberghaus, Jan wrote: > > Dear flowers: > > I am cloning human T cells specific for viral antigens and have slightly > confusing result now when I phenotype the clones. They are all CD3+, all > CD8+, all CD4-, all HLA-DR+ and now comes the confusing part 10-30% positive > for CD56 and 5-10% CD16 positive (the last stimulation with irrradiated > feeders and PHA is 12 days ago, the cells are cultured in IL-2 at 1000 U/ml). > > What are these cells: 1) remaining feeders? 2) my cultures are not clonal? 3) > in vitro phenomenon because of culture condition and stimulation? 4) NKT > cells? > > Help with resolving this is appreciated > Cheers > Jan Frederic I. Preffer Department of Pathology Charlestown Navy Yard- 7140 Massachusetts General Hospital East Charlestown, MA 02129 voice [617] 726-7481 fax [617] 724-3164
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