Hello Victoria, Neutrophils are certainly a challenge. A good reference is: "Granulocyte Storage and Antigen Stability," by H. Chun, K. Cipplone, J. Procter, and D.F. Stroncek in Transfusion vol 39, September 1999, pages 983-990. Regards, Nan Cochrane Neutrophil Serology Laboratory North Central Blood Services American Red Cross St. Paul, MN -----Original Message----- From: vbsmith@UNMC.EDU [mailto:vbsmith@UNMC.EDU] Sent: Wednesday, March 14, 2001 10:51 AM To: cyto-inbox Subject: degranulation? Hi, I have a question. The question is: What do people do to prevent peripheral blood neutrophils from degranulating during handling and storage. Are there special reagents, media, or buffers that can be used? Thank you in advance, Victoria Boryca Smith UNMC vbsmith@unmc.edu
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