At 05:34 15/3/2001, Mark Kukuruga wrote: >Hi all . . . >Considering the use of DAPI as an exclusion dye, I'm wondering effects of >about the (albeit brief) UV irradiation on the cells that we sort >"viably." Should we worry, or is it negligible? >MAK. The same question would apply to sorting viable cells stained for DNA with Hoescht, I've heard of this being done for a long time; anyone got experience to relate? After-effects could either result from UV exposure or from the dye binding direct to DNA; if there are effects, how would you decide which cause? Joseph.
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