PI signal in FL3

From: Juan Oliver M.D. (jao7@columbia.edu)
Date: Wed Mar 14 2001 - 19:22:29 EST 

I have an embryonic pluripotent cell line that has a subpopulation of
cells
with a spontaneous strong signal with a FACScalibur in FL3 (7AAD); the
remarkable finding is that this population of cells increases its signal
one log in
FL3 after PI addition (FL2); 10 micoliters of 0.1 mg/ml into ~ 1ml .
Nobody in our facility can explain it; any suggestions would be
appreciated.  Thanks, Juan Oliver

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