I have a client who has been valiantly trying to use the Biosource Int. Anti ERK 1/2(pTpY1851187) FITC labeled antibody to measure MAP-K. She is using freshly isolated human PBMC's. The Biosource booklet shows a nice overlay of histograms with Jurkat cells, both unstimultated and PMA stimulated. The PMA stimulated histogram being (what appears to be) much greater. The booklet states that fresh human PBMC's can also be used in this manner. She is seeing no difference in her stimulated and unstimulated samples. When she calls to talk to the company she is not getting much help. I am wondering if anyone is using this asay and if you would have any suggestions or ideas. Thanks in advance, Joan K Flow Cytometry Lab Supervisor VMRCVM (540) 231-4115 FAX 540-231-7367 jkalnits@vt.edu "It is better to serve than to receive." B. Borg
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