The following paper might be useful. "Differentiation of A31T6 Proadipocytes to Adipocytes: A Flow Cytometric Analysis"; Miriam Smith & Walker Wharton; in Experimental Cell Research 199, 29-38 (1992) Robb At 10:14 AM 2/21/01 -0600, Julie Pribyl wrote: >I have an investigator who wants to look at rat adipocytes by flow. >Has anyone performed this analysis? I'm a bit confused about the >references I found. One paper in Cytometry ( Bernstein et al >10:469-474, 1989) provides a lot of detail and it looks like this >isn't a simple procedure. More recently another paper performed >adipocyte analsyis on a FACSCan (Teruel et al, Biochem. J., >310:771-776, 1995). > >Any thoughts or suggestions? >JULIE >-- >*********************************** >Julie A. R. Pribyl >University of Minnesota Cancer Center >Coordinator, Flow Cytometry Core Facility/LeBien Lab >Phone: 612-624-7151 >e-mail: rehma001@tc.umn.edu >http://www.cancer.umn.edu/page/cores/flowcyte.html >*********************************** Robert C. Habbersett Technical Staff Member BioScience Division BN2, MS-M888 Los Alamos National Laboratory Los Alamos, NM 87545 505.667.0296 office/lab 505.665.3024 fax
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